Course Detail
Bot-601 MOLECULAR BIOLOGY 3(2+1)
Theory: Maximum Mark: 75 Practical: Maximum Mark: 25
Theory
1. Nucleic Acids: DNA-circular and superhelical DNA. Renaturation, Hybridization, sequencing of nucleic acid, synthesis of DNA
2. Proteins: Basic features of protein molecules. Folding of polypeptide Chain, a-helical and a-secondary structures. Protein purification and Sequencing.
3. Transcription: Enzymatic synthesis of RNA, transcriptional signals Translation: The genetic code. The Wobbling, polycistronic and monocistronic RNA. Overlapping genes.
4. Gene regulation in Eukaryotes: Difference in genetic organization and Prokaryotes and eukaryotes. Regulation of transcription, initiation, regulation of RNA processing, regulation of nucleocytoplasmic mRNA transport, regulation of mRNA stability, regulation of translation, regulation of protein activity.
5. Plant Omics: Transcriptomics; DNA libraries, their construction, Screening and application. Microarray of gene technology and its application in functional genomic.
6. Proteomics; structural and functional proteomics. Method to study Proteomics Metabolomics; methods to study metabolomics; Importance and application of metabolomics.
7. Bioinformatics and computational biology. Levels, scope, potential and Industrial application of bioinformatics and computational biology.
Practicals:
Following techniques will be used for the isolation and analysis of different components:
1. Extraction of RNA, DNA and proteins
2. Electrophoreses: One and two dimensional
3. Purification of proteins, RNA and DNA.
4. Amplification using PCR.
5. Northern, Western and Southern Blotting.
Books Recommended
1. Cullis, C.A. 2004. Plant Genomics and Proteomics. Wiley-Liss, New York.
2. Gibson, G. and S.V. Muse, 2002. A Premier of Genome Science, Sinauer Associates Inc. Massachusetts.
3. Gilmartin, P.M. and C. Bowler. 2002. Molecular plant biology. Vol. 1 & Oxford University Press, UK.
4. Lodish, H. et al., 2004. Molecular Cell Biology. 5th Edition. W.H. Freeman & Co., New York.
5. Malacinski, G. M. 2003. Essentials of Molecular Biology, 4th Edition.Jones and Bartlett Publishers, Massachusetts.
6. Watson, J.D. et al. 2004. Molecular Biology of the Gene. Peason Education, Singapore.
7. Ignacimuthu, S. 2005. Basic bioinformation. Narosa Publishing House, India.
8. Weaver, R.F. 2005. Molecular Biology. Mc|Graw Hill, St. Louis.